FAQ#510 Version1

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How many mismatches are allowed for mapping the reads against the human genome?

Updated by Subramanian, Sai Lakshmi about 10 years ago.

Category: - Difficluty: Difficluty5
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Related version:- Valid:Valid

Answer

The smallRNA-seq pipeline uses the sRNAbench tool to align reads to the human genome.
sRNAbench uses a default setting noMM=1, which means the number of mismatches
used by bowtie1 is 1

For further information on default settings used by sRNAbench,
please refer to the sRNAbench manual