FAQ#507 Version2

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How many reads should we have minimum to be able to cover low abundant targets with confidence in small RNA sequencing from plasma samples?

Updated by Thistlethwaite, William over 7 years ago.

Category: smallRNA Pipeline Difficluty: Difficluty5
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Answer

About 1-10 million usable reads per sample (after filtering rRNAs, calibrators, etc.) is ideal to get a good profile of the sequenced small RNAs.