FAQ#503 Version1
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What are the various output files generated by the pipeline?
Category: | smallRNA Pipeline | Difficluty: | |
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Related version: | - | Valid: | Valid |
Answer
The pipeline generates a variety of results and intermediate files, all of these are
compressed into a single archive AnalysisName_results.tar.gz
and uploaded to your output database.
Here is a description of the various output files:
- Read length distribution - File with extension
.clipped.readLengths.txt
- .stats - Summary statistics of read counts mapped to various libraries
- short_reads.txt - reads that are too small to be considered (<15nt by default)
- reads_orig.fa - adapter clipped, collapsed reads that are within the required length
- reads.fa & readsNG.fa - these seem to be intermediate files, I do not ever use these
- reads_withAdapter.fa - should always be empty as adapters are removed
- genome.parsed - the result of the read mapping to the host genome
- reads.annotation - these are all the reads mapped to the host genome in terms of their corresponding smallRNA annotation
- hairpin/ subdirectory - read alignments against the hairpin sequences
You can find more information on other output files generated by sRNABench in the attached tutorial PDF.