FAQ#503 Version1

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What are the various output files generated by the pipeline?

Updated by Subramanian, Sai Lakshmi over 10 years ago.

Category: smallRNA Pipeline Difficluty: Difficluty5
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Answer

The pipeline generates a variety of results and intermediate files, all of these are
compressed into a single archive AnalysisName_results.tar.gz and uploaded to your output database.

Here is a description of the various output files:

  • Read length distribution - File with extension .clipped.readLengths.txt
  • .stats - Summary statistics of read counts mapped to various libraries
  • short_reads.txt - reads that are too small to be considered (<15nt by default)
  • reads_orig.fa - adapter clipped, collapsed reads that are within the required length
  • reads.fa & readsNG.fa - these seem to be intermediate files, I do not ever use these
  • reads_withAdapter.fa - should always be empty as adapters are removed
  • genome.parsed - the result of the read mapping to the host genome
  • reads.annotation - these are all the reads mapped to the host genome in terms of their corresponding smallRNA annotation
  • hairpin/ subdirectory - read alignments against the hairpin sequences

You can find more information on other output files generated by sRNABench in the attached tutorial PDF.