FAQ#501 Version2
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Can I upload 3' adapter clipped input files?
| Category: | smallRNA Pipeline | Difficluty: |  |
| Assigned to: | - | Due date: | |
| Related issue: | - | Related Message: | - |
| Related version: | - | Valid: | Valid |
Answer
Yes, the pipeline accepts 3' adapter clipped input files.
- If you know the 3' adapter sequence for this sample, it is highly recommended to provide that adapter sequence in the 3' Adapter Sequence text box.
- However, the pipeline has a step that finds 3' adapter sequence in the input FASTQ file and removes it, so it is NOT required to provide an adapter-clipped input file. If the 3' adapter sequence is already clipped in your input FASTQ file, then check the box Clipped Input FASTQ file in the Tool Settings dialog while submitting the job.