Prepare Your longRNAseq Data Archive

  • Your data files must all be FASTQ paired-end sequencing read files.
  • It is acceptable for individual FASTQ files to be compressed.
  • If you wish to include a spike-in FASTA file, that file should also be included in your data archive.

Step 1. Gather All of Your Data Files in the Same Directory

  • Move all of your data files (FASTQ) into the same directory.
  • Optionally, you can also include a FASTA file with spike-in sequences for your samples.
    • You cannot include multiple spike-in sequence files. Only one FASTA file is allowed.

Step 2. Compress Data Files into One Archive

  • Place all data files into a single archive.
    • The archive must be .tar.gz or .zip format.
  • The data archive's file name must end in _longRNAseq_data.
    • For example, "" would be valid. So would "exRNA_longRNAseq_data.tar.gz".
  • If you need help creating an archive, please visit the Creating an Archive page.
  • IMPORTANT: If you are creating your archive on a Mac, please create a .tar.gz and not a .zip.
    We have run into some issues with decompressing large zip archives that were created using the Mac archiving software.


  1. Gather all of your data files in the same directory (including spike-in file, if necessary)
  2. Compress data files into a single archive

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