ENCODE ChIP-seq data Treatment Description¶
Treatment |
Description |
Label |
None |
No special treatment or protocol applies |
No treatment or prot |
4OHTAM_1uM_36hr |
(To replace TAM_1uM_36hr) 36 h with 1 uM 4-hydroxytamoxifen (Myers) |
4-OHTAM 1uM 36hr |
DMSO_0.02pct_24hr |
24 h with 0.02% Dimethyl sufloxide (DMSO) (Myers) |
DMSO_0.02pct_24hr |
EtOH_0.02pct |
1 h with 0.02% Ethanol (Myers) |
EtOH 0.02% 1h |
BPA_100nM_4hr |
4 h with 100 nM Bisphenol A (Myers) |
BPA_100nM_4hr |
DEX_100nM |
1 h with 100 nM Dexamethasone (Myers) |
DEX_100nM |
DEX_100pM |
1 h with 100 pM Dexamethasone (Myers) |
DEX_100pM |
DEX_1nM |
1 h with 1 nM Dexamethasone (Myers) |
DEX_1nM |
DEX_500pM |
1 h with 500 pM Dexamethasone (Myers) |
DEX_500pM |
DEX_5nM |
1 h with 5 nM Dexamethasone (Myers) |
DEX_5nM |
DMSO_4hr |
4 hr with 0.02% Dimethyl sufloxide (DMSO) (Myers) |
DMSO_4hr |
Estradiol_10nM_4hr |
4 h with 10 nM Estradiol (Myers) |
Estradiol_10nM_4hr |
EtOH_0.02pct |
1 h with 0.02% Ethanol (Myers) |
EtOH 0.02% 1h |
Genistein_100nM_4hr |
4 h with 100 nM Genistein (Myers) |
Genistein_100nM_4 |
ENCODE ChIP-seq data Protocol Description¶
Protocol |
Description |
Label |
PCR1x |
one 15-cycle round of PCR (Myers) |
PCR 1-round |
PCR2x |
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers) |
PCR 2-round |
v041610.1 |
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers) |
biorupter, PCR 1-round |
v041610.2 |
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers) |
sonicator, PCR 1-round |
v042211.1 |
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers) |
ChIP AMpure XP |
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