FAQ#467 Version1

Version 1/1 - Current version

What are Entry Points?

Updated by Riehle, Kevin about 12 years ago.

Category: Getting Started with Genboree Difficluty: Difficluty4
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Answer

What are Entry Points?

  • Entry points comprise the coordinate system on which annotations are made.
    • For example, a chromosome is an entry point. For unassembled genomes, the scaffolds might be the entry points.
  • Entry points must be defined within Genboree, with a name and a length, so that annotations can be placed on them.
  • Currently, entry points are independent within Genboree; i.e. not directly linked. They are simply the names of chromosomes
Uploading entry points:
  • Entry points can be uploaded in two different formats:
    • 3-column LFF
      • The entry point name
      • The keyword "Chromosome"
      • The length of the entry point
    • Fasta files
Helpful links:

Real Example

As an example, you can download the chromosome sizes for Mouse MM10 from UCSC:

We can turn this into a 3 column LFF format for uploading onto Genboree with some very light formatting:

chr1    Chromosome    195471971
chr2    Chromosome    182113224
chrX    Chromosome    171031299
chr3    Chromosome    160039680
chr4    Chromosome    156508116
chr5    Chromosome    151834684
chr6    Chromosome    149736546
chr7    Chromosome    145441459
chr10    Chromosome    130694993
chr8    Chromosome    129401213
chr14    Chromosome    124902244
chr9    Chromosome    124595110
chr11    Chromosome    122082543
chr13    Chromosome    120421639
chr12    Chromosome    120129022
chr15    Chromosome    104043685
chr16    Chromosome    98207768
chr17    Chromosome    94987271
chrY    Chromosome    91744698
chr18    Chromosome    90702639
chr19    Chromosome    61431566
chr5_JH584299_random    Chromosome    953012
chrX_GL456233_random    Chromosome    336933
chrY_JH584301_random    Chromosome    259875
chr1_GL456211_random    Chromosome    241735
chr4_GL456350_random    Chromosome    227966
chr4_JH584293_random    Chromosome    207968
chr1_GL456221_random    Chromosome    206961
chr5_JH584297_random    Chromosome    205776
chr5_JH584296_random    Chromosome    199368
chr5_GL456354_random    Chromosome    195993
chr4_JH584294_random    Chromosome    191905
chr5_JH584298_random    Chromosome    184189
chrY_JH584300_random    Chromosome    182347
chr7_GL456219_random    Chromosome    175968
chr1_GL456210_random    Chromosome    169725
chrY_JH584303_random    Chromosome    158099
chrY_JH584302_random    Chromosome    155838
chr1_GL456212_random    Chromosome    153618
chrUn_JH584304    Chromosome    114452
chrUn_GL456379    Chromosome    72385
chr4_GL456216_random    Chromosome    66673
chrUn_GL456393    Chromosome    55711
chrUn_GL456366    Chromosome    47073
chrUn_GL456367    Chromosome    42057
chrUn_GL456239    Chromosome    40056
chr1_GL456213_random    Chromosome    39340
chrUn_GL456383    Chromosome    38659
chrUn_GL456385    Chromosome    35240
chrUn_GL456360    Chromosome    31704
chrUn_GL456378    Chromosome    31602
chrUn_GL456389    Chromosome    28772
chrUn_GL456372    Chromosome    28664
chrUn_GL456370    Chromosome    26764
chrUn_GL456381    Chromosome    25871
chrUn_GL456387    Chromosome    24685
chrUn_GL456390    Chromosome    24668
chrUn_GL456394    Chromosome    24323
chrUn_GL456392    Chromosome    23629
chrUn_GL456382    Chromosome    23158
chrUn_GL456359    Chromosome    22974
chrUn_GL456396    Chromosome    21240
chrUn_GL456368    Chromosome    20208
chrM    Chromosome    16299
chr4_JH584292_random    Chromosome    14945
chr4_JH584295_random    Chromosome    1976