The c.13217T>C variant in USH2A is a missense variant predicted to cause substitution of leucine by proline at amino acid 4406 (p.Leu4406Pro). The highest population minor allele frequency in gnomAD v4.1.0 is 0.02% (15/74934) in the African / African American population (PM2_supporting, BS1, and BA1 not met). The computational predictor REVEL gives a score of 0.338, which is neither above nor below the thresholds predicting a damaging or benign impact on USH2A function. This variant has been identified in one individual with Usher syndrome who was compound heterozygous with a pathogenic variant phase unknown (PP4, 0.5 point PM3; PMID: 36011402). This individual also had a third variant that was reviewed by the Hearing Loss VCEP and was determined to be likely benign. This variant was also identified in an individual with retinitis pigmentosa who was compound heterozygous with a pathogenic variant, though phase was unknown (0.5 point PM3, PMID: 37217489). The variant was also reported in other individuals with Usher syndrome; however, these individuals were not awarded any evidence as zygosity / phasing was unknown, the second variant was likely benign, or the individual had a possible alternate explanation for disease (PMID: 26927203, 24944099, 36011402). In summary, this variant has been classified as a variant of uncertain significance for autosomal recessive Usher syndrome based on the ACMG/AMP criteria applied, as specified by the ClinGen Hearing Loss VCEP: PM3, PP4 (ClinGen Hearing Loss VCEP specifications version 2; 11.20.2024).