alias: Histone.DS21539
expected_number_runs: 2
center_name: NREMC

STUDY_REF: UwStam_NREMC_CA
SAMPLE_DESCRIPTOR: fThymus.H-24644d110

LIBRARY
LIBRARY_NAME: Histone.DS21539
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: multiplexed libraries
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 36

BASE CALLS
RTA 1.13

QUALITY SCORES
RTA 1.13

ATTRIBUTES
EXPERIMENT_TYPE: Histone H3K4me3
EXTRACTION_PROTOCOL: http://www.roadmapepigenomics.org/protocols/type/experimental
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 20-40 cycles of 0.5 minute on and 0.5 minute off
CHIP_PROTOCOL: http://www.roadmapepigenomics.org/protocolstype/experimental
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 60ug
CHIP_PROTOCOL_BEAD_TYPE: Invitrogen Dynabeads
CHIP_PROTOCOL_BEAD_AMOUNT: 60ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 12ul
CHIP_ANTIBODY: Histone H3K4me3
CHIP_ANTIBODY_PROVIDER: Cell Signaling
CHIP_ANTIBODY_CATALOG: 9751B
CHIP_ANTIBODY_LOT: lot 2