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Reference Epigenome: ChIP-Seq Analysis of H3T11ph in hESC H9 Cells
ChIP-Seq Analysis of H3T11ph in hESC H9 Cells. Sequencing was done on the Illumina Genome Analyzer IIx platform.
Project News:
2011/9/28: EXPERIMENT XML file 'renlab.H3T11ph.hESC.H9.01.01.experiment.xml' uploaded
alias: renlab.H3T11ph.hESC.H9.01.01
expected_number_runs: 1 center_name: UCSD STUDY_REF: The San Diego Epigenome Center SAMPLE_DESCRIPTOR: SRS184463 LIBRARY LIBRARY_NAME: SK509 LIBRARY_STRATEGY: ChIP-Seq LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: ChIP LIBRARY_LAYOUT: SINGLE POOLING_STRATEGY: none LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ILLUMINA INSTRUMENT_MODEL: Illumina Genome Analyzer IIx SEQUENCE_LENGTH: 36 BASE CALLS Illumina Sequencing Control Software 2.8 QUALITY SCORES Illumina Sequencing Control Software 2.8 ATTRIBUTES EXPERIMENT_TYPE: Histone H3T11ph EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Biorupter EXTRACTION_PROTOCOL_SONICATION_CYCLES: 80 CHIP_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms CHIP_PROTOCOL_BEAD_TYPE: magnetic anti-rabbit CHIP_PROTOCOL_BEAD_AMOUNT: 33,500,000 CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 micrograms CHIP_ANTIBODY: H3T11ph CHIP_ANTIBODY_PROVIDER: Active Motif CHIP_ANTIBODY_CATALOG: 39152 CHIP_ANTIBODY_LOT: 258 |
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