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Shotgun sequencing of polyA+ RNA isolated from the H1 cell line differentiated into neural progenitor cells
Shotgun sequencing of polyA+ RNA isolated from the H1 cell line differentiated into neural progenitor cells
Project News:
2011/3/29: EXPERIMENT XML file 'ecker.mRNA-Seq_20110330.experiment.xml' uploaded
alias: mRNA-Seq_h1-npc_r2
expected_number_runs: 1 center_name: UCSD STUDY_REF: The San Diego Epigenome Center SAMPLE_DESCRIPTOR: H1-NPC_r2 LIBRARY LIBRARY_NAME: mRNA-Seq_h1-npc_r2 LIBRARY_STRATEGY: mRNA-Seq LIBRARY_SOURCE: TRANSCRIPTOMIC LIBRARY_SELECTION: cDNA LIBRARY_LAYOUT: SINGLE POOLING_STRATEGY: none LIBRARY_CONSTRUCTION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ABI_SOLID INSTRUMENT_MODEL: AB SOLiD 4 System COLOR_MATRIX: COLOR_MATRIX_CODE: Standard SEQUENCE_LENGTH: 50 BASE CALLS Standard SOLiD 4 installation NA QUALITY SCORES Standard SOLiD 4 installation NA ATTRIBUTES EXPERIMENT_TYPE: mRNA-Seq EXTRACTION_PROTOCOL: RNeasy Blood and Tissue Kit (Qiagen) EXTRACTION_PROTOCOL_MRNA_ENRICHMENT: Poly(A)Purist MAG Kit (Ambion) EXTRACTION_PROTOCOL_FRAGMENTATION: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) MRNA_PREPARATION_INITIAL_MRNA_QNTY: 20-100 ng MRNA_PREPARATION_FRAGMENT_SIZE_RANGE: 200 nt RNA_PREPARATION_5'_RNA_ADAPTER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) RNA_PREPARATION_3'_RNA_ADAPTER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) RNA_PREPARATION_5'_DEPHOSPHORYLATION: NA RNA_PREPARATION_5'_PHOSPHORYLATION: NA RNA_PREPARATION_3'_RNA ADAPTER_LIGATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) RNA_PREPARATION_5'_RNA_ADAPTER_LIGATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_TEMPLATE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_NUMBER_CYCLES: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_PRIMER_CONC: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies) |
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