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Reference Epigenome: ChIP-Seq Input from hESC H1 Cells
ChIP-Seq Input from hESC H1 Cells. Sequencing was done on the Illumina GAIIx platform.


Project News:
2010/10/4:
EXPERIMENT XML file 'renlab.Input.hESC-05.01.experiment.xml' uploaded



alias: renlab.Input.hESC-05.01
expected_number_runs: 1
expected_number_spots: 15342951
expected_number_reads: 15342951
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: H1-04

LIBRARY
LIBRARY_NAME: YL154
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: RANDOM
LIBRARY_LAYOUT: SINGLE
LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

SPOT
NUMBER_OF_READS_PER_SPOT: 1
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer IIx
CYCLE_SEQUENCE: ACGT
CYCLE_COUNT: 36

BASE CALLS
SEQUENCE_SPACE: Base Space
BASE_CALLER: Illumina Sequencing Control Software version 2.8

QUALITY SCORES
Quality Type: phred
QUALITY_SCORER: Illumina Sequencing Control Software version 2.8
NUMBER_OF_LEVELS: 80
MULTIPLIER: 1

ATTRIBUTES
EXPERIMENT_TYPE: ChIP-Seq Input
EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Branson Tip Sonicator
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 30
CHIP_PROTOCOL: Input
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms

 

 


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