alias: renlab.H3K27ac.STL003SG.02.01
expected_number_runs: 1
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: SRS306624

LIBRARY
LIBRARY_NAME: AY94
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: PAIRED
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

SPOT
NUMBER_OF_READS_PER_SPOT: 2
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1
READ_INDEX: 2
READ_CLASS: Application Read
READ_TYPE: Reverse
BASE_COORD: 77

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 100

BASE CALLS
Illumina HiSeq Control Software 2.0.12.0

QUALITY SCORES
Illumina HiSeq Control Software 2.0.12.0

ATTRIBUTES
EXPERIMENT_TYPE: Histone H3K27ac
EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Biorupter
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 80
CHIP_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms
CHIP_PROTOCOL_BEAD_TYPE: magnetic anti-rabbit
CHIP_PROTOCOL_BEAD_AMOUNT: 33,500,000
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 micrograms
CHIP_ANTIBODY: H3K27ac
CHIP_ANTIBODY_PROVIDER: Active Motif
CHIP_ANTIBODY_CATALOG: 39133
CHIP_ANTIBODY_LOT: 19208002