alias: STL002_genome
expected_number_runs: 1
center_name: Salk

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: STL002

LIBRARY
LIBRARY_NAME: STL002
LIBRARY_STRATEGY: WGS
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: RANDOM
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: multiplexed samples
LIBRARY_CONSTRUCTION_PROTOCOL: Two µg of genomic DNA was extracted from ground, frozen tissue using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA). The DNA was fragmented with a Covaris S2 (Covaris, Woburn, MA) to 300-400 bp, followed by library preparation using the TruSeq DNA Sample Prep kit (Illumina, San Diego, CA) as per manufacturer's instructions. The library was run on a 2% agarose gel and gel size selected to 400-500 bp using the MinElute Gel Extraction kit (Qiagen).

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 100

BASE CALLS
N/A N/A

QUALITY SCORES
N/A N/A

ATTRIBUTES
EXPERIMENT_TYPE: Whole Genome Sequencing
EXTRACTION_PROTOCOL: Qiagen DNeasy Blood and Tissue kit, performed as per manufacturer's instructions
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Covaris S2
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 400 bp target peak size, 2 cycles