alias: renlab.H3K9me3.IMR90-01.01
expected_number_runs: 4
expected_number_spots: 49824825
expected_number_reads: 49824825
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: IMR90_r1

LIBRARY
LIBRARY_NAME: LL224
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

SPOT
SPOT_DECODE_SPEC: 1 0 Application Read Forward 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer II
CYCLE_SEQUENCE: ACGT
CYCLE_COUNT: 36

BASE CALLS
SEQUENCE_SPACE: Base Space
BASE_CALLER: Illumina Genome Analyzer Pipeline Software version 1.3.2

QUALITY SCORES
Quality Type: phred
QUALITY_SCORER: Illumina Genome Analyzer Pipeline Software version 1.3.2
NUMBER_OF_LEVELS: 80
MULTIPLIER: 1

ATTRIBUTES
EXPERIMENT_TYPE: Histone H3K9me3
EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Branson Tip Sonicator
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 30
CHIP_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms
CHIP_PROTOCOL_BEAD_TYPE: magnetic anti-rabbit
CHIP_PROTOCOL_BEAD_AMOUNT: 33,500,000
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 micrograms
CHIP_ANTIBODY: H3K9me3
CHIP_ANTIBODY_PROVIDER: Abcam
CHIP_ANTIBODY_CATALOG: ab8898
CHIP_ANTIBODY_LOT: 484099