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ChIP-Seq analysis of H3K9me3 in human CD25int CD127+ Tmem cells
Human Chromatin IP REMC Sequencing on Illumina
Project News:
2011/9/29: EXPERIMENT XML file 'BI.experiment.G1828.2011-09-29.xml' uploaded
alias: DNA_Lib 737
center_name: BI expected_number_runs: 3 STUDY_REF: G1828 SAMPLE_DESCRIPTOR: SRS255292 LIBRARY LIBRARY_NAME: DNA_Lib 737 LIBRARY_STRATEGY: ChIP-Seq LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: ChIP LIBRARY_LAYOUT: SINGLE POOLING_STRATEGY: multiplexed libraries LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ILLUMINA INSTRUMENT_MODEL: Illumina HiSeq 2000 SEQUENCE_LENGTH: 36 BASE CALLS GAPipeline RTA1.12.4.1 QUALITY SCORES GAPipeline RTA1.12.4.1 ATTRIBUTES CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B CHIP_ANTIBODY: H3K9me3 CHIP_PROTOCOL_CHROMATIN_AMOUNT: 0.5 to 3 million cells EXTRACTION_PROTOCOL: cell/nuclei lysis CHIP_PROTOCOL_ANTIBODY_AMOUNT: ~1 ug CHIP_PROTOCOL_BEAD_AMOUNT: 10 ul bed volume CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1 CHIP_ANTIBODY_CATALOG: ab8898 CHIP_ANTIBODY_PROVIDER: Abcam CHIP_ANTIBODY_LOT: 877391 EXPERIMENT_TYPE: Histone H3K9me3 EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Branson |
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