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ChIP-Seq analysis of H3K4me1 in human HUES48 cells
Human Chromatin IP REMC Sequencing on Illumina
Project News:
2011/7/26: EXPERIMENT XML file 'BI.experiment.G1828.2011-07-21.xml' uploaded
alias: Lib EpiLabSPL Row 1713
center_name: BI expected_number_runs: 1 STUDY_REF: G1828 SAMPLE_DESCRIPTOR: 201 LIBRARY LIBRARY_NAME: Lib EpiLabSPL Row 1713 LIBRARY_STRATEGY: ChIP-Seq LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: ChIP LIBRARY_LAYOUT: SINGLE POOLING_STRATEGY: none LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ILLUMINA INSTRUMENT_MODEL: Illumina Genome Analyzer IIx SEQUENCE_LENGTH: 36 BASE CALLS GAPipeline 1.8.0 QUALITY SCORES GAPipeline 1.8.0 ATTRIBUTES CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B CHIP_ANTIBODY: H3K4Me1 CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells EXTRACTION_PROTOCOL: SDS lysis CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1 CHIP_ANTIBODY_CATALOG: ab8895 CHIP_ANTIBODY_PROVIDER: Abcam CHIP_ANTIBODY_LOT: 659352 EXPERIMENT_TYPE: Histone H3K4me1 EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor |
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