Genboree BCM
Help Genboree Home
(This is a recently added feature. Report issues to Genboree Admin.)
ChIP-Seq analysis of H3K36me3 in human BM-MSC cells
Human Chromatin IP REMC Sequencing on Illumina


Project News:
2011/1/30:
EXPERIMENT XML file 'BI.experiment.G2303.2011-01-30.xml' uploaded



alias: Lib:MC:20100713:10--ChIP:MC:20100706:10:BM-MSC:H3K36Me3
center_name: BI
expected_number_runs: 1

STUDY_REF: G2303
SAMPLE_DESCRIPTOR: 78

LIBRARY
LIBRARY_NAME: Lib:MC:20100713:10--ChIP:MC:20100706:10:BM-MSC:H3K36Me3
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer IIx
SEQUENCE_LENGTH: 36

BASE CALLS
GAPipeline 1.8.0

QUALITY SCORES
GAPipeline 1.8.0

ATTRIBUTES
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K36Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells
EXTRACTION_PROTOCOL: SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: ab9050
CHIP_ANTIBODY_PROVIDER: Abcam
CHIP_ANTIBODY_LOT: 499302
EXPERIMENT_TYPE: Histone H3K36me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor

 

 


Bioinformatics Research Laboratory © 2001-2024 Baylor College of Medicine
Bioinformatics Research Laboratory
(400D Jewish Wing, MS:BCM225, 1 Baylor Plaza, Houston, TX 77030)