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ChIP-Seq analysis of H3K9me3 in human hES-I3 cells
Human Chromatin IP REMC Sequencing on Illumina
Project News:
2010/3/3: EXPERIMENT XML file 'BI.experiment.42M0GAAXX.1.hES-I3.H3K9me3.xml' uploaded
alias: 42M0GAAXX091022-1-S
center_name: BI expected_number_reads: 10000000 expected_number_runs: 1 expected_number_spots: 10000000 STUDY_REF: G1828 SAMPLE_DESCRIPTOR: 40586.0 LIBRARY LIBRARY_NAME: Solexa-15475 LIBRARY_STRATEGY: ChIP-Seq LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: ChIP LIBRARY_LAYOUT: SINGLE LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina SPOT NUMBER_OF_READS_PER_SPOT: 1 READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ILLUMINA INSTRUMENT_MODEL: Illumina Genome Analyzer II CYCLE_SEQUENCE: CYCLE_COUNT: 36 BASE CALLS SEQUENCE_SPACE: Base Space BASE_CALLER: Solexa primary analysis QUALITY SCORES Quality Type: other QUALITY_SCORER: Solexa primary analysis NUMBER_OF_LEVELS: 80 MULTIPLIER: 1.0 ATTRIBUTES EXPERIMENT_TYPE: Histone H3K9me3 EXTRACTION_PROTOCOL: SDS lysis EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1 CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug CHIP_ANTIBODY: H3K9Me3 CHIP_ANTIBODY_PROVIDER: Abcam CHIP_ANTIBODY_CATALOG: ab8898 CHIP_ANTIBODY_LOT: 484088 |
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