expected_number_runs: 1
center_name: NREMC
alias: RNA.RS19855

STUDY_REF: UwStam_NREMC_CA
SAMPLE_DESCRIPTOR: SRS267039

LIBRARY
LIBRARY_NAME: lib-RNA.RS19855
LIBRARY_STRATEGY: mRNA-Seq
LIBRARY_SOURCE: TRANSCRIPTOMIC
LIBRARY_SELECTION: cDNA
LIBRARY_LAYOUT: PAIRED
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: Illumina TruSeq RNA PART# 15008136 REVISION A

SPOT
NUMBER_OF_READS_PER_SPOT: 1
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 36

BASE CALLS
Illumina RTA 1.12.4.2

QUALITY SCORES
Illumina RTA 1.12.4.2

ATTRIBUTES
EXPERIMENT_TYPE: mRNA-Seq
EXTRACTION_PROTOCOL: Ambion mirVana miRNA isolation kit
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
RNA_PREPARATION_INITIAL_RNA_QNTY: 1ug
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE: Random Primers(hexamers)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL: TruSeq RNA
LIBRARY_GENERATION_PCR_TEMPLATE: cDNA
LIBRARY_FRAGMENTATION: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
LIBRARY_FRAGMENT_SIZE_RANGE: 120-200bp
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: Proprietary
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: 98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
LIBRARY_GENERATION_PCR_NUMBER_CYCLES: 15
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: Proprietary
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: Proprietary
LIBRARY_GENERATION_PCR_PRIMER_CONC: Proprietary
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit