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ChIP-Seq Input of Fetal Muscle, Leg
ChIP-Seq Input


Project News:
2012/12/27:
EXPERIMENT XML file 'NREMC.experiment.Histone.input.DS21509.xml' uploaded



alias: Histone.DS21509
expected_number_runs: 1
center_name: NREMC

STUDY_REF: UwStam_NREMC_CA
SAMPLE_DESCRIPTOR: fMuscle_Leg.H-24644d110

LIBRARY
LIBRARY_NAME: Histone.DS21509
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: RANDOM
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: multiplexed libraries
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 36

BASE CALLS
RTA 1.13

QUALITY SCORES
RTA 1.13

ATTRIBUTES
EXPERIMENT_TYPE: ChIP-Seq Input
EXTRACTION_PROTOCOL: http://www.roadmapepigenomics.org/protocols/type/experimental
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 20-40 cycles of 0.5 minute on and 0.5 minute off
CHIP_PROTOCOL: Input
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 50ng

 

 


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