alias: HS0996
expected_number_runs: 1
center_name: BCCAGSC
expected_number_spots: 8966624
expected_number_reads: 8966624

STUDY_REF: UCSF_UBC_HREMP
SAMPLE_DESCRIPTOR: HS0995

LIBRARY
LIBRARY_NAME: H1EScd1-me3K4-A
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
LIBRARY_CONSTRUCTION_PROTOCOL: Farnham lab protocol

SPOT
SPOT_DECODE_SPEC: 1 0 Application Read Forward 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer II
CYCLE_SEQUENCE: ACGT
CYCLE_COUNT: 50

BASE CALLS
SEQUENCE_SPACE: Base Space
BASE_CALLER: Bustard 1.3.2

QUALITY SCORES
Quality Type: phred
QUALITY_SCORER: Illumina pipeline 1.3.2
NUMBER_OF_LEVELS: 80
MULTIPLIER: 1

ATTRIBUTES
EXTRACTION_PROTOCOL: n/a
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 15 min
CHIP_PROTOCOL: Farnham lab protocol
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 44ug
CHIP_PROTOCOL_BEAD_TYPE: Protein G Mag Beads
CHIP_PROTOCOL_BEAD_AMOUNT: 30uL (CST)
CHIP_PROTOCOL_ANTIBODY_AMOUNT: n/a
CHIP_ANTIBODY: H3K4me3
CHIP_ANTIBODY_PROVIDER: Cell Signaling Technology
CHIP_ANTIBODY_CATALOG: 9751
CHIP_ANTIBODY_LOT: 1