expected_number_runs: 1
center_name: BCCAGSC
alias: A03480-1

STUDY_REF: UCSF_UBC_HREMP
SAMPLE_DESCRIPTOR: SRS167234

LIBRARY
LIBRARY_NAME: A03480
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: BCCAGSC ChIP Standard Operating Procedure

SPOT
NUMBER_OF_READS_PER_SPOT: 1
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 76

BASE CALLS
Bustard 1.9.0

QUALITY SCORES
Bustard 1.9.0

ATTRIBUTES
EXPERIMENT_TYPE: Histone H3K27me3
EXTRACTION_PROTOCOL: BCCAGSC ChIP Standard Operating Procedure
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Fisher 550 Sonic Dismembrator
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 10min
CHIP_PROTOCOL: BCCAGSC ChIP Standard Operating Procedure
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 5ug
CHIP_PROTOCOL_BEAD_TYPE: Sepharose A/G beads
CHIP_PROTOCOL_BEAD_AMOUNT: 20ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5ug
SIZE_FRACTION: 85-379bp
CHIP_ANTIBODY: H3K27me3
CHIP_ANTIBODY_PROVIDER: Diagenode
CHIP_ANTIBODY_CATALOG: pAb069050
CHIP_ANTIBODY_LOT: A29900241