expected_number_runs: 1
center_name: BCCAGSC
alias: A09692-1

STUDY_REF: UCSF_UBC_HREMP
SAMPLE_DESCRIPTOR: SRS265435

LIBRARY
LIBRARY_NAME: A09692
LIBRARY_STRATEGY: MeDIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: 5-methylcytidine antibody
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: Costello Lab MeDIP Protocol

SPOT
NUMBER_OF_READS_PER_SPOT: 1
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1
READ_INDEX: 1
READ_CLASS: Technical Read
READ_TYPE: BarCode
BASE_COORD: 77

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 84

BASE CALLS
Illumina RTA 1.12.4.2

QUALITY SCORES
Illumina RTA 1.12.4.2

ATTRIBUTES
EXPERIMENT_TYPE: DNA Methylation
EXTRACTION_PROTOCOL: Costello Lab MeDIP Protocol
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 30min
MeDIP_PROTOCOL: Costello Lab MeDIP Protocol
MeDIP_PROTOCOL_DNA_AMOUNT: 5ug
MeDIP_PROTOCOL_BEAD_TYPE: Protein A/G
MeDIP_PROTOCOL_BEAD_AMOUNT: 100ul
MeDIP_PROTOCOL_ANTIBODY_AMOUNT: 800ng
MeDIP_ANTIBODY: Methylcytidine
MeDIP_ANTIBODY_PROVIDER: Eurogentec
MeDIP_ANTIBODY_CATALOG: BI-MECY-0100
MeDIP_ANTIBODY_LOT: 80808