alias: renlab.H4K20me1.hESC.H1.02.01
expected_number_runs: 1
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: SRS167138

LIBRARY
LIBRARY_NAME: SK529
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer IIx
SEQUENCE_LENGTH: 36

BASE CALLS
Illumina Sequencing Control Software 2.9

QUALITY SCORES
Illumina Sequencing Control Software 2.9

ATTRIBUTES
EXPERIMENT_TYPE: Histone H4K20me1
EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Biorupter
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 80
CHIP_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms
CHIP_PROTOCOL_BEAD_TYPE: magnetic anti-rabbit
CHIP_PROTOCOL_BEAD_AMOUNT: 33,500,000
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 micrograms
CHIP_ANTIBODY: H4K20me1
CHIP_ANTIBODY_PROVIDER: Active Motif
CHIP_ANTIBODY_CATALOG: 39727
CHIP_ANTIBODY_LOT: 3610001