alias: mRNA-Seq_h1-npc_r2
expected_number_runs: 1
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: H1-NPC_r2

LIBRARY
LIBRARY_NAME: mRNA-Seq_h1-npc_r2
LIBRARY_STRATEGY: mRNA-Seq
LIBRARY_SOURCE: TRANSCRIPTOMIC
LIBRARY_SELECTION: cDNA
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ABI_SOLID
INSTRUMENT_MODEL: AB SOLiD 4 System
COLOR_MATRIX:
COLOR_MATRIX_CODE: Standard
SEQUENCE_LENGTH: 50

BASE CALLS
Standard SOLiD 4 installation NA

QUALITY SCORES
Standard SOLiD 4 installation NA

ATTRIBUTES
EXPERIMENT_TYPE: mRNA-Seq
EXTRACTION_PROTOCOL: RNeasy Blood and Tissue Kit (Qiagen)
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT: Poly(A)Purist MAG Kit (Ambion)
EXTRACTION_PROTOCOL_FRAGMENTATION: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
MRNA_PREPARATION_INITIAL_MRNA_QNTY: 20-100 ng
MRNA_PREPARATION_FRAGMENT_SIZE_RANGE: 200 nt
RNA_PREPARATION_5'_RNA_ADAPTER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_3'_RNA_ADAPTER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_5'_DEPHOSPHORYLATION: NA
RNA_PREPARATION_5'_PHOSPHORYLATION: NA
RNA_PREPARATION_3'_RNA ADAPTER_LIGATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_5'_RNA_ADAPTER_LIGATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_TEMPLATE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_NUMBER_CYCLES: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_PRIMER_CONC: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)