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Whole genome sequencing of the IMR90 cell line
Whole genome sequencing of the IMR90 cell line


Project News:
2013/10/19:
EXPERIMENT XML file 'ecker.IMR90.Genome.experimental.xml' uploaded



alias: IMR90-05_Genome_PE
expected_number_runs: 1
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: SRS024804

LIBRARY
LIBRARY_NAME: IMR90-05_Genome_PE
LIBRARY_STRATEGY: WGS
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: RANDOM
LIBRARY_LAYOUT: PAIRED
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: Genomic DNA was extracted from frozen cell pellets using the DNeasy Mini Kit (Qiagen, Valencia, CA). The DNA was used to create one fragmented DNA library as described in the ?Applied Biosystems SOLiDTM 4 System Library Preparation Guide? (Applied Biosystems, Foster City, CA).

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ABI_SOLID
INSTRUMENT_MODEL: AB SOLiD 4 System
SEQUENCE_LENGTH: 100

BASE CALLS
Illumina Genome Analyzer Pipeline Software 1.4

QUALITY SCORES
Illumina Genome Analyzer Pipeline Software 1.4

ATTRIBUTES
EXPERIMENT_TYPE: Whole Genome Sequencing
EXTRACTION_PROTOCOL: Qiagen DNeasy Mini kit, performed as per manufacturer's instructions
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Covaris S2
EXTRACTION_PROTOCOL_SONICATION_CYCLES: Standard fragment express, 6 cycles

 

 


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