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Whole genome sequencing of the IMR90 cell line
Whole genome sequencing of the IMR90 cell line
Project News:
2013/10/19: EXPERIMENT XML file 'ecker.IMR90.Genome.experimental.xml' uploaded
alias: IMR90-05_Genome_PE
expected_number_runs: 1 center_name: UCSD STUDY_REF: The San Diego Epigenome Center SAMPLE_DESCRIPTOR: SRS024804 LIBRARY LIBRARY_NAME: IMR90-05_Genome_PE LIBRARY_STRATEGY: WGS LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: RANDOM LIBRARY_LAYOUT: PAIRED POOLING_STRATEGY: none LIBRARY_CONSTRUCTION_PROTOCOL: Genomic DNA was extracted from frozen cell pellets using the DNeasy Mini Kit (Qiagen, Valencia, CA). The DNA was used to create one fragmented DNA library as described in the ?Applied Biosystems SOLiDTM 4 System Library Preparation Guide? (Applied Biosystems, Foster City, CA). SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ABI_SOLID INSTRUMENT_MODEL: AB SOLiD 4 System SEQUENCE_LENGTH: 100 BASE CALLS Illumina Genome Analyzer Pipeline Software 1.4 QUALITY SCORES Illumina Genome Analyzer Pipeline Software 1.4 ATTRIBUTES EXPERIMENT_TYPE: Whole Genome Sequencing EXTRACTION_PROTOCOL: Qiagen DNeasy Mini kit, performed as per manufacturer's instructions EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Covaris S2 EXTRACTION_PROTOCOL_SONICATION_CYCLES: Standard fragment express, 6 cycles |
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