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Reference Epigenome: ChIP-Seq Analysis of H3K27me3 in Human Sigmoid Colon Tissue
ChIP-Seq Analysis of H3K27me3 in Human Sigmoid Colon Tissue. Sequencing was done on the Illumina Genome Analyzer IIx platform.


Project News:
2012/4/8:
EXPERIMENT XML file 'renlab.H3K27me3.STL001SG.01.01.experiment.xml' uploaded



alias: renlab.H3K27me3.STL001SG.01.01
expected_number_runs: 1
center_name: UCSD

STUDY_REF: The San Diego Epigenome Center
SAMPLE_DESCRIPTOR: STL001SG-01

LIBRARY
LIBRARY_NAME: AK230
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer IIx
SEQUENCE_LENGTH: 36

BASE CALLS
Illumina Sequencing Control Software 2.9

QUALITY SCORES
Illumina Sequencing Control Software 2.9

ATTRIBUTES
EXPERIMENT_TYPE: Histone H3K27me3
EXTRACTION_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Biorupter
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 80
CHIP_PROTOCOL: See http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 500 micrograms
CHIP_PROTOCOL_BEAD_TYPE: magnetic anti-rabbit
CHIP_PROTOCOL_BEAD_AMOUNT: 33,500,000
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 micrograms
CHIP_ANTIBODY: H3K27me3
CHIP_ANTIBODY_PROVIDER: Active Motif
CHIP_ANTIBODY_CATALOG: 39155
CHIP_ANTIBODY_LOT: 30508004

 

 


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