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ChIP-Seq analysis of H3K27ac in human CD25- CD45RA+ naive cells
Human Chromatin IP REMC Sequencing on Illumina


Project News:
2011/7/30:
EXPERIMENT XML file 'BI.experiment.G1828.2011-07-29.xml' uploaded



alias: DNA_Lib 730
center_name: BI
expected_number_runs: 4

STUDY_REF: G1828
SAMPLE_DESCRIPTOR: BioSam 133

LIBRARY
LIBRARY_NAME: DNA_Lib 730
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: multiplexed libraries
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina HiSeq 2000
SEQUENCE_LENGTH: 36

BASE CALLS
GAPipeline RTA1.12.4.1

QUALITY SCORES
GAPipeline RTA1.12.4.1

ATTRIBUTES
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K27ac
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 0.5 to 3 million cells
EXTRACTION_PROTOCOL: cell/nuclei lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: ~1 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 10 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: ab4729
CHIP_ANTIBODY_PROVIDER: Abcam
CHIP_ANTIBODY_LOT: 509313
EXPERIMENT_TYPE: Histone H3K27ac
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Branson

 

 


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