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ChIP-Seq analysis of H3K9me3 in human AdiposeNuclei cells
Human Chromatin IP REMC Sequencing on Illumina


Project News:
2011/1/30:
EXPERIMENT XML file 'BI.experiment.G2303.2011-01-30.xml' uploaded



alias: Lib:XZ:20100731:06--ChIP:XZ:20100726:06:AdiposeNuclei:H3K9Me3
center_name: BI
expected_number_runs: 1

STUDY_REF: G2303
SAMPLE_DESCRIPTOR: 140

LIBRARY
LIBRARY_NAME: Lib:XZ:20100731:06--ChIP:XZ:20100726:06:AdiposeNuclei:H3K9Me3
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
POOLING_STRATEGY: none
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT:
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer IIx
SEQUENCE_LENGTH: 36

BASE CALLS
GAPipeline 1.8.0

QUALITY SCORES
GAPipeline 1.8.0

ATTRIBUTES
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K9Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells
EXTRACTION_PROTOCOL: SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: ab8898
CHIP_ANTIBODY_PROVIDER: Abcam
CHIP_ANTIBODY_LOT: 484088
EXPERIMENT_TYPE: Histone H3K9me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor

 

 


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