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ChIP-Seq analysis of H3K4me3 in human Mobilized CD34 cells
Human Chromatin IP REMC Sequencing on Illumina


Project News:
2010/10/5:
EXPERIMENT XML file 'BI.experiment.G2303.2010-10-05.xml' uploaded



alias: Lib:RI:20100524:03--ChIP:MA:20100517:03:Mobilized CD34:H3K4Me3
center_name: BI
expected_number_reads: 10000000
expected_number_runs: 1
expected_number_spots: 10000000

STUDY_REF: G2303
SAMPLE_DESCRIPTOR: UW DS # 14129

LIBRARY
LIBRARY_NAME: Lib:RI:20100524:03--ChIP:MA:20100517:03:Mobilized CD34:H3K4Me3
LIBRARY_STRATEGY: ChIP-Seq
LIBRARY_SOURCE: GENOMIC
LIBRARY_SELECTION: ChIP
LIBRARY_LAYOUT: SINGLE
LIBRARY_CONSTRUCTION_PROTOCOL: Single read - Illumina

SPOT
NUMBER_OF_READS_PER_SPOT: 1
READ_INDEX: 0
READ_CLASS: Application Read
READ_TYPE: Forward
BASE_COORD: 1

PLATFORM
ILLUMINA
INSTRUMENT_MODEL: Illumina Genome Analyzer II
CYCLE_SEQUENCE:
CYCLE_COUNT: 36

BASE CALLS
SEQUENCE_SPACE: Base Space
BASE_CALLER: Solexa primary analysis

QUALITY SCORES
Quality Type: other
QUALITY_SCORER: Solexa primary analysis
NUMBER_OF_LEVELS: 80
MULTIPLIER: 1.0

ATTRIBUTES
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K4Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells
EXTRACTION_PROTOCOL: SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: 07-473
CHIP_ANTIBODY_PROVIDER: Millipore
CHIP_ANTIBODY_LOT: DAM1623866
EXPERIMENT_TYPE: Histone H3K4me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor

 

 


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