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Affymetrix HG-U133 arrays of NIH Epigenomics Roadmap Cell Types
Primary tissue from skeletal muscle was isolated from surgical samples; total RNA was isolated and subject to Affymetrix profiling
Project News:
2012/6/29:
EXPERIMENT XML file 'BI.expression_array.experiment.G1828.2012-06-29.xml' uploaded
alias: RNAPrp 28_632062
center_name: BI
STUDY_REF: G1828
SAMPLE_DESCRIPTOR: SRS120867
ATTRIBUTES
EXTRACT_PROTOCOL: Cells were lysed and RNA extracted using Qiagen Rneasy kit
LABEL_PROTOCOL: As recommended by Affymetrix
PROBE_GROUP_FILE: not applicable to 3' array platform
HYB_PROTOCOL: As recommended by Affymetrix
TREATMENT_PROTOCOL: untreated primary tissue
GROWTH_PROTOCOL: Uncultured primary tissue
META-PROBESET_FILE: not applicable to 3' array platform
VALUE_DEFINITION: signal calculated by GeneChIP Command Console software
SCAN_PROTOCOL: As recommended by Affymetrix
DATA_PROCESSING: Data were processed using Affymetrix GeneChIP Command Console, algorithm version 1.0.0.1029
PLATFORM: Affymetrix HG-U133_Plus_2
EXPERIMENT_TYPE: Expression Array
center_name: BI
STUDY_REF: G1828
SAMPLE_DESCRIPTOR: SRS120867
ATTRIBUTES
EXTRACT_PROTOCOL: Cells were lysed and RNA extracted using Qiagen Rneasy kit
LABEL_PROTOCOL: As recommended by Affymetrix
PROBE_GROUP_FILE: not applicable to 3' array platform
HYB_PROTOCOL: As recommended by Affymetrix
TREATMENT_PROTOCOL: untreated primary tissue
GROWTH_PROTOCOL: Uncultured primary tissue
META-PROBESET_FILE: not applicable to 3' array platform
VALUE_DEFINITION: signal calculated by GeneChIP Command Console software
SCAN_PROTOCOL: As recommended by Affymetrix
DATA_PROCESSING: Data were processed using Affymetrix GeneChIP Command Console, algorithm version 1.0.0.1029
PLATFORM: Affymetrix HG-U133_Plus_2
EXPERIMENT_TYPE: Expression Array
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