|
|||||||||
Bisulfite-Seq analysis of RRBS_Lib 270 derived from human inferior temporal lobe cells
RRBS REMC Sequencing on Illumina
Project News:
2012/3/21: EXPERIMENT XML file 'BI.experiment.G1828.2012-03-21.xml' uploaded
alias: RRBS_Lib 270
center_name: BI expected_number_runs: 2 STUDY_REF: G1828 SAMPLE_DESCRIPTOR: SRS255293 LIBRARY LIBRARY_NAME: RRBS_Lib 270 LIBRARY_STRATEGY: Bisulfite-Seq LIBRARY_SOURCE: GENOMIC LIBRARY_SELECTION: Reduced Representation LIBRARY_LAYOUT: SINGLE POOLING_STRATEGY: multiplexed libraries LIBRARY_CONSTRUCTION_PROTOCOL: Smith et al., Methods 48, 226-232 SPOT NUMBER_OF_READS_PER_SPOT: READ_INDEX: 0 READ_CLASS: Application Read READ_TYPE: Forward BASE_COORD: 1 PLATFORM ILLUMINA INSTRUMENT_MODEL: Illumina HiSeq 2000 SEQUENCE_LENGTH: 29 BASE CALLS GAPipeline RTA1.12.4.2 QUALITY SCORES GAPipeline RTA1.12.4.2 ATTRIBUTES BISULFITE_CONVERSION_PROTOCOL: 2x5hrs Epitect Kit DNA_PREPARATION_INITIAL_DNA_QNTY: 30-300 ng EXTRACTION_PROTOCOL_SONICATION_CYCLES: NA DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL: T4 Ligase (2,000U/ul) 16C o/n DNA_PREPARATION_ADAPTOR_SEQUENCE: Illumina TruSeq adapter LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: Illumina TruSeq primer DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION: 120-400 BISULFITE_CONVERSION_PERCENT: 99.5% EXTRACTION_PROTOCOL: Standard Protocol (Smith et al., Methods 48, 226-232) LIBRARY_GENERATION_PCR_PRIMER_CONC: 25uM DNA_PREPARATION_FRAGMENT_SIZE_RANGE: 30-280 EXPERIMENT_TYPE: DNA Methylation LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: Smith et al., Methods 48, 226-232 LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: SPRI Beads LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: Illumina TruSeq primer pair LIBRARY_GENERATION_PCR_TEMPLATE_CONC: NA LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: Pfu Turbo Cx hot start LIBRARY_GENERATION_PCR_NUMBER_CYCLES: 11-15 EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: NA |
|||||||||
|
|||||||||